The purpose of an ELISA is to determine if a particular protein is present in a sample and if so, how much. There are two main variations on this method: you can determine how much antibody is in a sample, or you can determine how much protein is bound by an antibody. The distinction is whether you are trying to quantify an antibody or some other protein. In this example, we will use an ELISA to determine how much of a particular antibody is present in an individuals blood.

ELISAs are performed in 96-well plates which permits high throughput results. The bottom of each well is coated with a protein to which will bind the antibody you want to measure. Whole blood is allowed to clot and the cells are centrifuged out to obtain the clear serum with antibodies (called primary antibodies). The serum is incubated in a well, and each well contains a different serum (see figure below). A positive control serum and a negative control serum would be included among the 96 samples being tested.

http://www.landging.com/skeletal-system-animation-aci-surgery.html
Designed for medical research organization, this skeletal system animation demonstrates the new concept of ACI (autologous chondrocyte implantation) surgery procedure.

http://www.landging.com/plane-animation.html
420 seconds 3d plane animation, designed for Expo 2010 Shanghai Aviation Pavilion.

http://www.landging.com/accident-animation-slip-and-fall.html
This accident animation demonstrates injuries caused by slip and fall.

http://www.landging.com/animated-city.html
420 seconds 3d animation which is developed for Expo 2010 Shanghai Aviation Pavilion and demonstrates an animated city.

http://www.landging.com/expo2010_case_2.html
Airplane perspective animation, 3d aviation animation, developed for Expo 2010 Shanghai Aviation Pavilion.

http://www.landging.com/underbalanced-drilling.html
This Underbalanced Drilling Animation demonstrates new oil drilling technology.

http://www.landging.com/well-test-animation.html
This well test animation demonstrates new oil drilling technology.

http://www.landging.com/through-tubing.html
This through-tubing perforation animation demonstrates new oil drilling technology.

http://www.landging.com/wireline-logging.html
This wireline logging animation demonstrates new oil drilling technology.

http://www.landging.com/annulus_pressure_responsive.html
This annulus pressure responsive (APR) animation demonstrates new oil drilling technology.

http://www.landging.com/accident-animation-workers-compensation-lifting-box.html
This lifting box accident animation demonstrates the injury covered by workers compensation program.

http://www.landging.com/accident-animation-workers-compensation-falling-stuff.html
This falling stuff accident animation demonstrates the injury covered by workers compensation program.

http://www.landging.com/accident-animation-workers-compensation-desk-job.html
This desk job accident animation demonstrates the injury covered by workers compensation program.

http://www.landging.com/accident-animation-sports-injury-yoga.html
This accident animation demonstrates sports injury in yoga practice.

http://www.landging.com/accident-animation-sports-injury-soccer.html
This accident animation demonstrates sports injury in soccer game.

http://www.landging.com/car-crash-animations-rear.html
Car crash animations, accident reconstruction, rear end collision.

http://www.landging.com/circulatory-system-animation-cardiology.html
This circulatory system animation demonstrates cardiology mechanism of action and pharmacology in 3d.

http://www.landging.com/breast-enlargement-animation.html
Breast enlargement product animation designed for TV shopping.

http://www.landging.com/respiratory-circulatory-system-animation.html
Human body circulatory system and respiratory system, 3D MOA (Mechanism of Action) animation, designed for Beijing Natural History Museum.