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Histological staining: hematoxylin & eosin
Histological staining: hematoxylin & eosin DrPhil 56 Views • 2 years ago

The most popular and one of the principal stains in histology is hematoxylin and eosin stain. It gives us an overview of the tissue and its structure. Hematoxylin binds with basophilic structures – for example DNA and RNA. So we can observe nuclei stained in blue or purple color. Eosin binds to acidophilic substances such as positively charged amino acid side chains. So as the result cytoplasm is pink or orange. All samples in laboratory are stained with H&E. There are several different types of hematoxylins and eosins used in histology which will give us different results.

In this video you will see, how we stain slides with different types of hematoxylins and eosins. Finally, we will compare the results.

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Cancer Care
Cancer Care News Canada 8,291 Views • 2 years ago

Doctor shares tips on what to expect after a cancer diagnosis

Diabetes and your eyes
Diabetes and your eyes samer kareem 4,833 Views • 2 years ago

Diabetic retinopathy involves changes to retinal blood vessels that can cause them to bleed or leak fluid, distorting vision. Diabetic retinopathy is the most common cause of vision loss among people with diabetes and a leading cause of blindness among working-age adults.

Right side abdominal pain
Right side abdominal pain samer kareem 6,514 Views • 2 years ago

Upper right quadrant: The right upper quadrant contains the liver and gallbladder, which are protected by the lower right part of the ribcage. The large intestine, or colon, also spends a little time in this section. Upper left quadrant: The left upper quadrant contains part of the stomach and the spleen.

Acanthosis Nigricans Insulin Resistance
Acanthosis Nigricans Insulin Resistance Medical_Videos 6,943 Views • 2 years ago

Acanthosis Nigricans Insulin Resistance

Acupuncture Weight Loss
Acupuncture Weight Loss Medical_Videos 7,895 Views • 2 years ago

Acupuncture Weight Loss

Psoriatic Arthritis
Psoriatic Arthritis samer kareem 2,655 Views • 2 years ago

Psoriatic arthritis is a chronic arthritis. In some people, it is mild, with just occasional flare ups. In other people, it is continuous and can cause joint damage if it is not treated. Early diagnosis is important to avoid damage to joints. Psoriatic arthritis typically occurs in people with skin psoriasis, but it can occur in people without skin psoriasis, particularly in those who have relatives with psoriasis. Psoriatic arthritis typically affects the large joints, especially those of the lower extremities, distal joints of the fingers and toes, and also can affect the back and sacroiliac joints of the pelvis. For most people, appropriate treatments will relieve pain, protect the joints, and maintain mobility. Physical activity helps maintain joint movement. Psoriatic arthritis is sometimes misdiagnosed as gout, rheumatoid arthritis or osteoarthritis. - See more at: http://www.rheumatology.org/I-Am-A/Patient-Caregiver/Diseases-Conditions/Psoriatic-Arthritis#sthash.VsBTUw76.dpuf

Anatomy of The Brain
Anatomy of The Brain Anatomy_Videos 32,441 Views • 2 years ago

Anatomy of The Brain

Anatomy of The Infratemporal Fossa
Anatomy of The Infratemporal Fossa Anatomy_Videos 7,787 Views • 2 years ago

Anatomy of The Infratemporal Fossa

Forzest 20mg for men| uses| result| action| side effects| by MedstoreRx.com
Forzest 20mg for men| uses| result| action| side effects| by MedstoreRx.com Jose Bell 2,174 Views • 2 years ago

Forzest is FDA approved medicine, it is used bt men to improve erectile dysfunction dusring intercourse session with partner. for more information related side effects, dosage, etc kindly visit to http://www.medstorerx.com/forzest.aspx

Anatomy of The Superficial Dissection of The Upper and Lower Limbs
Anatomy of The Superficial Dissection of The Upper and Lower Limbs Anatomy_Videos 17,916 Views • 2 years ago

Anatomy of The Superficial Dissection of The Upper and Lower Limbs

Laparoscopic Appendicectomy Surgery Video
Laparoscopic Appendicectomy Surgery Video Mohamed Ibrahim 22,291 Views • 2 years ago

What is the Appendix? The appendix is a long narrow tube (a few inches in length) that attaches to the first part of the colon. It is usually located in the lower right quadrant of the abdominal cavity. The appendix produces a bacteria destroying protein called immunoglobulins, which help fight infection in the body. Its function, however, is not essential. People who have had appendectomies do not have an increased risk toward infection. Other organs in the body take over this function once the appendix has been removed. What is a Laparoscopic Appendectomy? Appendicitis is one of the most common surgical problems. One out of every 2,000 people has an appendectomy sometime during their lifetime. Treatment requires an operation to remove the infected appendix. Traditionally, the appendix is removed through an incision in the right lower abdominal wall. In most laparoscopic appendectomies, surgeons operate through 3 small incisions (each ¼ to ½ inch) while watching an enlarged image of the patient’s internal organs on a television monitor. In some cases, one of the small openings may be lengthened to complete the procedure. Advantages of Laparoscopic Appendectomy Results may vary depending upon the type of procedure and patient’s overall condition. Common advantages are: Less postoperative pain May shorten hospital stay May result in a quicker return to bowel function Quicker return to normal activity Better cosmetic results Are You a Candidate for Laparoscopic Appendectomy? Although laparoscopic appendectomy has many benefits, it may not be appropriate for some patients. Early, non-ruptured appendicitis usually can be removed laparoscopically. Laparoscopic appendectomy is more difficult to perform if there is advanced infection or the appendix has ruptured. A traditional, open procedure using a larger incision may be required to safely remove the infected appendix in these patients.

Brain Concussion Recognize and Report
Brain Concussion Recognize and Report Surgeon 6,533 Views • 2 years ago

Brain Concussion Recognize and Report

Anatomy of the Human Abdominal Wall
Anatomy of the Human Abdominal Wall samer kareem 3,360 Views • 2 years ago

Clinical Anatomy Lecture Illustrate The Anatomy Of The Abdominal Wall

What a Sight!
What a Sight! News Canada 6,760 Views • 2 years ago

Take a trip around the world while ensuring healthy eye habits and optimal vision

Huge Colon Polyp Removal
Huge Colon Polyp Removal Surgeon 15,129 Views • 2 years ago

Huge Colon Polyp Removal

Sports Medicine Animation / Soccer
Sports Medicine Animation / Soccer Landging 5,640 Views • 2 years ago

http://www.landging.com/accident-animation-sports-injury-soccer.html
This accident animation demonstrates sports injury in soccer game.

Balloon Sinuplasty and Endoscopic Sinus Surgery
Balloon Sinuplasty and Endoscopic Sinus Surgery samer kareem 13,924 Views • 2 years ago

Sinusitis and Sinus Surgery Explained (Balloon Sinuplasty and Endoscopic Sinus Surgery)

Difference between HIV and AIDS
Difference between HIV and AIDS Magdy 9,160 Views • 2 years ago

Human immunodeficiency virus infection / acquired immunodeficiency syndrome (HIV/AIDS) is a disease of the human immune system caused by the human immunodeficiency virus (HIV).[1] During the initial infection a person may experience a brief period of influenza-like illness. This is typically followed by a prolonged period without symptoms. As the illness progresses it interferes more and more with the immune system, making people much more likely to get infections, including opportunistic infections, and tumors that do not usually affect people with working immune systems.
HIV is transmitted primarily via unprotected sexual intercourse (including anal and even oral sex), contaminated blood transfusions and hypodermic needles, and from mother to child during pregnancy, delivery, or breastfeeding.[2] Some bodily fluids, such as saliva and tears, do not transmit HIV.[3] Prevention of HIV infection, primarily through safe sex and needle-exchange programs, is a key strategy to control the spread of the disease. There is no cure or vaccine; however, antiretroviral treatment can slow the course of the disease and may lead to a near-normal life expectancy. While antiretroviral treatment reduces the risk of death and complications from the disease, these medications are expensive and may be associated with side effects.
Genetic research indicates that HIV originated in West-central Africa during the early twentieth century.[4] AIDS was first recognized by the Centers for Disease Control and Prevention (CDC) in 1981 and its cause—HIV infection—was identified in the early part of the decade.[5] Since its discovery, AIDS has caused nearly 30 million deaths (as of 2009).[6] As of 2010, approximately 34 million people have contracted HIV globally.[7] AIDS is considered a pandemic—a disease outbreak which is present over a large area and is actively spreading.[8]
HIV/AIDS has had a great impact on society, both as an illness and as a source of discrimination. The disease also has significant economic impacts. There are many misconceptions about HIV/AIDS such as the belief that it can be transmitted by casual non-sexual contact. The disease has also become subject to many controversies involving religion.

ELISA
ELISA Magdy 5,796 Views • 2 years ago

The purpose of an ELISA is to determine if a particular protein is present in a sample and if so, how much. There are two main variations on this method: you can determine how much antibody is in a sample, or you can determine how much protein is bound by an antibody. The distinction is whether you are trying to quantify an antibody or some other protein. In this example, we will use an ELISA to determine how much of a particular antibody is present in an individuals blood.

ELISAs are performed in 96-well plates which permits high throughput results. The bottom of each well is coated with a protein to which will bind the antibody you want to measure. Whole blood is allowed to clot and the cells are centrifuged out to obtain the clear serum with antibodies (called primary antibodies). The serum is incubated in a well, and each well contains a different serum (see figure below). A positive control serum and a negative control serum would be included among the 96 samples being tested.

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