H&E stain is a popular staining method in histology. Its a combination of two dyes: the basic dye (hematoxylin) and the alcohol-based dye (eosin). In an H&E stain you will usually see both eosinophilia and basophilia: the nuclei of cells basophilic (blue), while eosinophilia is typical of cytoplasmic constituents (pink). Xylene, alcohols, distilled water are also required.

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Glaucoma is called the silent thief of sight. It does not have symptoms during the early stages of the diseases and can make a patient blind over several years

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The purpose of an ELISA is to determine if a particular protein is present in a sample and if so, how much. There are two main variations on this method: you can determine how much antibody is in a sample, or you can determine how much protein is bound by an antibody. The distinction is whether you are trying to quantify an antibody or some other protein. In this example, we will use an ELISA to determine how much of a particular antibody is present in an individuals blood.

ELISAs are performed in 96-well plates which permits high throughput results. The bottom of each well is coated with a protein to which will bind the antibody you want to measure. Whole blood is allowed to clot and the cells are centrifuged out to obtain the clear serum with antibodies (called primary antibodies). The serum is incubated in a well, and each well contains a different serum (see figure below). A positive control serum and a negative control serum would be included among the 96 samples being tested.

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Recent studies show that administration of PEA in glaucoma patients has a double effect, decrease the IOP and neuroprotection. The IOP is the major risk factor in glaucoma, constricts blood vessels and reduces the delivery of oxygen and nutrients to the retina and optic nerve (ON), causing a process of ischemia and cell death (apoptosis).
New research points out palmitoylethanolamide has a dual action in glaucoma:

1. It reduces high eye pressure by promoting fluid flow out of the eye, and

2. PEA protects nerve cells and retina cells via its neuroprotective and reparative properties.

In the Youtube the essence of the natural treatment of glaucoma with palmitoylethanolamide has been summarized. Daily dose: 2-3 times 400 mg palmitoylethanolamide. Literature on this topic on http://palmitoylethanolamide4pain.com/2015/02/20/youtube-on-palmitoylethanolamide-as-a-natural-treatment-for-glaucoma/