Conductive System of the Heart

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The purpose of an ELISA is to determine if a particular protein is present in a sample and if so, how much. There are two main variations on this method: you can determine how much antibody is in a sample, or you can determine how much protein is bound by an antibody. The distinction is whether you are trying to quantify an antibody or some other protein. In this example, we will use an ELISA to determine how much of a particular antibody is present in an individuals blood.

ELISAs are performed in 96-well plates which permits high throughput results. The bottom of each well is coated with a protein to which will bind the antibody you want to measure. Whole blood is allowed to clot and the cells are centrifuged out to obtain the clear serum with antibodies (called primary antibodies). The serum is incubated in a well, and each well contains a different serum (see figure below). A positive control serum and a negative control serum would be included among the 96 samples being tested.

Alzheimers disease is an irreversible, progressive brain disease that slowly destroys memory and thinking skills

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How we went from super species to the sickest. What gravity has to do with it.

Evaluación de la microcirculación con SDF (Sidestream dark field) Microscan® como método de monitorización no invasiva en microcirugía.

Laser Hair Removal In Delhi at SCULPT Aesthetic & Cosmetic Clinic.

A subdural hematoma (SDH) is a collection of blood below the inner layer of the dura but external to the brain and arachnoid membrane (see the images below). Subdural hematoma is the most common type of traumatic intracranial mass lesion. Subdural hematoma occurs not only in patients with severe head injury but also in patients with less severe head injuries, particularly those who are elderly or who are receiving anticoagulants. Subdural hematoma may also be spontaneous or caused by a procedure, such as a lumbar puncture (see Etiology). Rates of mortality and morbidity can be high, even with the best medical and neurosurgical care (see Prognosis). Subdural hematomas are usually characterized on the basis of their size and location and the amount of time elapsed since the inciting event age (ie, whether they are acute, subacute, or chronic). When the inciting event is unknown, the appearance of the hematoma on neuroimaging studies can help determine when the hematoma occurred. These factors, as well as the neurologic and medical condition of the patient, determine the course of treatment and may also influence the outcome. Generally, acute subdural hematomas are less than 72 hours old and are hyperdense compared with the brain on computed tomography scans. The subacute phase begins 3-7 days after acute injury. Chronic subdural hematomas develop over the course of weeks and are hypodense compared with the brain. However, subdural hematomas may be mixed in nature, such as when acute bleeding has occurred into a chronic subdural hematoma. Presentation varies widely in acute subdural hematoma (see Clinical). Many of these patients are comatose on admission. However, approximately 50% of patients with head injuries who require emergency neurosurgery present with head injuries that are classified as moderate or mild (Glasgow Coma Scale scores 9-13 and 14-15, respectively). Many of these patients harbor intracranial mass lesions. In a large series of patients who developed intracranial hematomas requiring emergent decompression, more than half had lucid intervals and were able to make conversation between the time of their injury and subsequent deterioration. In a more comprehensive review of the literature on the surgical treatment of acute subdural hematomas, lucid intervals were noted in up to 38% of cases. These patients may be more likely to benefit from medical and surgical intervention when instituted in a timely fashion (ie, before further neurological deterioration).

Surgeons at The Children’s Hospital of Philadelphia were the first to perform a bilateral hand transplant on a child. Our research and work in this groundbreaking field of medicine led us to establish the Hand Transplantation Program. Combining the expertise of the Penn Transplant Institute and the Hospital’s Division of Plastic and Reconstructive Surgery and Division of Orthopedics, the program aims to improve quality of life for children who may benefit from this procedure.

Parkinson disease (PD) is a common neurodegenerative condition. Typically beginning in the sixth or seventh decade of life, it is characterized by the unilateral onset of resting tremor in combination with varying degrees of rigidity and bradykinesia. PD was originally described by James Parkinson (1755-1824), a man of many talents and interests. Parkinson published works on chemistry, paleontology, and other diverse topics. Early in his career he was a social activist championing the rights of the disenfranchised and poor. His efforts in this area were enough to result in his arrest and appearance before the Privy Council in London on at least one occasion. In collaboration with his son, who was a surgeon, he also offered the first description in the English language of a ruptured appendix. His small but famous publication, "Essay on the Shaking Palsy," was published in 1817, seven years before his death. The clinical descriptions of 6 cases was remarkable in part because he never actually examined the people he described. Instead, he had simply observed these people on the streets of London.