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In order to be able to look at tissues under a microscope, we need to first stain them with the right technique. Learn the main staining techniques used in histology today on our full video: https://khub.me/aux9w
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As you probably know, histology is the study of the microscopic anatomy of cells and tissues. So we use staining methods to visualize and distinguish the different parts of cells and tissues since cells and their structures are usually transparent or colorless. The types of dyes used to color cells and their components can either be specific to particular structures, chemical groups or even molecules, and it can also be non-specific in which case most of the cell is stained in the same way.
When staining tissue samples, dyes that are used are either acidic or basic or a combination of the two. And why is that, you might be asking. Well, cellular structures such as nucleic acids or proteins have charged groups which are known as phosphate groups or carboxyl groups, just to name a couple. The dyes used in histology are colored organic compounds which also have a charge. Acidic dyes carry a negative charge and so they bind to positively-charged cell structures.
In the full version of this tutorial, we will cover some of the most common types of dyes used in histological staining of cells and their structures:
- basic dyes vs acidic dyes vs neutral dyes;
- hematoxylin and eosin;
- PAS - staining;
- Golgi method;
- Toluidine blue;
- Masson's trichrome;
- Osmium tetroxide;
To master this topic, click on the link and carry on watching the full video (available to Premium members): https://khub.me/aux9w !
Want to test your knowledge on the different types of cells and tissues? Take this quiz: https://khub.me/3g19f
Read more on how to interpret different histological sections on this complete article which goes through the different stains used in histology https://khub.me/saimh
For more engaging video tutorials, interactive quizzes, articles and an atlas of Human anatomy and histology, go to https://khub.me/pkvz2
This is an example of a surgery to fix a femur (thigh bone) fracture utilizing an intramedullary nail. This is a minimally invasive way of fixing this surgical problem and allows for immediate range of motion and full weight-bearing.
A new report analyzing FDA-approved monoclonal antibodies (mAbs) produced by a select group of leading biotechnology companies shows that clinical development times – specifically the duration of Phase II and Phase III trials – are lengthening, while FDA review times have remained constant. The average time from investigational new drug (IND) filing to market was 6.7 years for 11 mABs approved between 1994 and 2003 but shot up to 8.3 years for 12 mAbs approved between 2004 and March 9, 2011, according to Deloitte Recap LLC’s analysis, Therapeutic Monoclonal Antibodies – Insights, Strategies and Data.
In today's video our patient is on the second stage of her breast reconstruction journey. Previously she had a mastectomy on the left side then we inserted a tissue expander to help stretch the breast tissue to create a pocket for the permanent breast implant that we are placing in today's video. On top of the breast implant we are grafting this patient's own fat into the breast to add a little extra volume and help it be more symmetrical with the other breast.
Mastectomy is surgery to remove all breast tissue from a breast as a way to treat or prevent breast cancer. For those with early-stage breast cancer, mastectomy may be one treatment option. Breast-conserving surgery (lumpectomy), in which only the tumor is removed from the breast, may be another option.
Symptoms of dizziness can result from many conditions such as; (vestibular) inner ear disorders, neck injuries or muscle tightness, neuropathy, central nervous system problems, metabolic issues, or psychological disorders. Our therapists are trained to screen for more serious conditions (such as neurological and cardiovascular disorders) as well as effectively evaluate and treat conditions which are appropriate for physical therapy intervention.