The purpose of an ELISA is to determine if a particular protein is present in a sample and if so, how much. There are two main variations on this method: you can determine how much antibody is in a sample, or you can determine how much protein is bound by an antibody. The distinction is whether you are trying to quantify an antibody or some other protein. In this example, we will use an ELISA to determine how much of a particular antibody is present in an individuals blood.

ELISAs are performed in 96-well plates which permits high throughput results. The bottom of each well is coated with a protein to which will bind the antibody you want to measure. Whole blood is allowed to clot and the cells are centrifuged out to obtain the clear serum with antibodies (called primary antibodies). The serum is incubated in a well, and each well contains a different serum (see figure below). A positive control serum and a negative control serum would be included among the 96 samples being tested.

Sickle cell anemia (sickle cell disease) is a disorder of the blood caused by an inherited abnormal hemoglobin (the oxygen-carrying protein within the red blood cells). The abnormal hemoglobin causes distorted (sickled) red blood cells.

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Simple interrupted suturing is the most basic and most important of the suturing techniques.

There is a new organ in your digestive system SHOW MORE

Umbilical Hernia Repair using the new device echnique Ventral Patch

A growing number of patients having total knee replacement surgery are 55 or younger. Surgeons at Sunnybrook's Holland Centre perform more than 1,000 total knee replacements each year. Read more: http://sunnyview.sunnybrook.ca..../2011/11/snap-crackl

Arterial line placement is a common procedure in various critical care settings. Intra-arterial blood pressure (BP) measurement is more accurate than measurement of BP by noninvasive means, especially in the critically ill. [1] Intra-arterial BP management permits the rapid recognition of BP changes that is vital for patients on continuous infusions of vasoactive drugs. Arterial cannulation also allows repeated arterial blood gas samples to be drawn without injury to the patient.

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#LaparoscopicColectomy #ColonSurgery #LargeIntestine

A colectomy is usually done to treat diseases that inflame your colon, a bowel obstruction, colon cancer, or a damaged or injured colon. The anatomy of the colon, and the laparoscopic procedure done to remove a portion of the colon, are depicted.

ANH18221

Primary biliary cholangitis (PBC), formerly known as primary biliary cirrhosis, is a chronic liver disease resulting from progressive destruction of the bile ducts in the liver – called the intrahepatic bile ducts. Bile produced in your liver travels via these ducts to your small intestine where it aids in the digestion of fat and fat-soluble vitamins (A, D, E and K). When the ducts are destroyed, bile builds up in the liver contributing to inflammation and scarring (fibrosis). Eventually this can lead to cirrhosis and its associated complications, as scar tissue replaces healthy liver tissue and liver function becomes increasingly impaired.

The gastrointestinal tract (GIT) arises initially during the process of gastrulation from the endoderm of the trilaminar embryo (week 3) and extends from the buccopharyngeal membrane to the cloacal membrane. The tract and associated organs later have contributions from all the germ cell layers. During the 4th week three distinct regions (fore-, mid- and hind-gut) extend the length of the embryo and will contribute different components of the GIT. The large mid-gut is generated by lateral embryonic folding which "pinches off" a pocket of the yolk sac, the 2 compartments continue to communicate through the vitelline duct. The oral cavity (mouth) is formed following breakdown of the buccopharyngeal membrane (oropharyngeal or oral membrane) and contributed to mainly by the pharynx lying within the pharyngeal arches (More? Head Development). Loss of buccopharyngeal membrane opens the tract to amniotic fluid through the remainder of development, and during the fetal period is actively swallowed.

The brain is made up of billions of nerve cells that are specially designed to communicate with each other. They form many connections with one another, creating an intricate network between cells.

How to Perform Pregnancy Test at Home Video

DMC Surgeon uses minimally-invasive surgery to remove uterine fibroids to hasten recovery. ~ Detroit Medical Center

In this educational clip, dr hosnani's rhinoplasty video is shown

A video showing an Exaggerated Knee Reflex

The video will describe what is miliary tuberculosis. Please see my website for disclaimer.

STOP VIOLENCE AGAINST DOCTORs

Arterial line insertion usually involves cannulation of the radial artery with a 20 gauge catheter for the purposes of beat-by-beat blood pressure monitoring all along with arterial blood gas monitoring.