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Basic histological staining methods (preview) - Human Histology | Kenhub DrPhil 34 Views • 2 years ago

In order to be able to look at tissues under a microscope, we need to first stain them with the right technique. Learn the main staining techniques used in histology today on our full video: https://khub.me/aux9w

Oh, are you struggling with learning anatomy? We created the ★ Ultimate Anatomy Study Guide ★ to help you kick some gluteus maximus in any topic. Completely free. Download yours today: https://khub.me/e0th1

As you probably know, histology is the study of the microscopic anatomy of cells and tissues. So we use staining methods to visualize and distinguish the different parts of cells and tissues since cells and their structures are usually transparent or colorless. The types of dyes used to color cells and their components can either be specific to particular structures, chemical groups or even molecules, and it can also be non-specific in which case most of the cell is stained in the same way.

When staining tissue samples, dyes that are used are either acidic or basic or a combination of the two. And why is that, you might be asking. Well, cellular structures such as nucleic acids or proteins have charged groups which are known as phosphate groups or carboxyl groups, just to name a couple. The dyes used in histology are colored organic compounds which also have a charge. Acidic dyes carry a negative charge and so they bind to positively-charged cell structures.

In the full version of this tutorial, we will cover some of the most common types of dyes used in histological staining of cells and their structures:
- basic dyes vs acidic dyes vs neutral dyes;
- hematoxylin and eosin;
- PAS - staining;
- Golgi method;
- Toluidine blue;
- Masson's trichrome;
- Osmium tetroxide;

To master this topic, click on the link and carry on watching the full video (available to Premium members): https://khub.me/aux9w !

Want to test your knowledge on the different types of cells and tissues? Take this quiz: https://khub.me/3g19f

Read more on how to interpret different histological sections on this complete article which goes through the different stains used in histology https://khub.me/saimh

For more engaging video tutorials, interactive quizzes, articles and an atlas of Human anatomy and histology, go to https://khub.me/pkvz2

Histology of Dentin DrPhil 42 Views • 2 years ago

The dentin is a hard tissue that forms the bulk of the tooth. It is similar to bone but is slightly harder, although softer than enamel. The dentin has numerous dentinal tubules that run across its length. Each dentinal tubule houses the cytoplasmic process of an odontoblast (odontoblastic process).
📄Notes for the video: https://www.hackdentistry.com/....bundles/revision-nin

💻Website: https://www.hackdentistry.com/
📰Blog: https://hackdentistry.substack.com/

Study resources on our website-
📖Oral pathology Revision Ninja (Notes, Videos & MCQs): https://www.hackdentistry.com/bundles/oral-pathology-revision-ninja
📖Oral Histology Revision Ninja (Notes, Videos & MCQs): https://www.hackdentistry.com/....bundles/revision-nin
📖Periodontics Revision Ninja (Notes & MCQs): https://www.hackdentistry.com/bundles/perio-rn
📖Question Bank: https://www.hackdentistry.com/bundles/question-bank
📖Access all content: https://www.hackdentistry.com/bundles/all-access-premium

References and further reading:
💡Berkovitz BKB, Hollan GR, Moxham BJ. Oral Anatomy, Histology and Embryology. 4th ed. Mosby Elsevier; 2009.
💡Nanci A. Tencate’s Oral Histology. Development, Structure and Function. 8th ed. Elsevier; 2013.
💡Kumar GS. Orban’s Oral Histology and Embryology.13th ed. Elsevier; 2011.
💡Avery JK. Oral development and Histology. 3rd ed. Thieme Medical Publishers; 2002.

Log in to https://www.hackdentistry.com and get access to:
I) Numerous Notes/Cheatsheets and Videos II) Thousands of quiz questions from our vast Question Bank!

HackDentistry is an edtech company that aims make learning dentistry fun,engaging and light hearted.
1) It focuses on helping students understand and retain core concepts in dentistry through highly visual sketch/whiteboard style video animations.
2) The platform helps improve exam performance by providing Revision Bundles and allowing students to test themselves using thousands of Practice Questions from a vast Question Bank. (multiple choice format).
3) It also provides for a community platform where students can come together, and engage with fellow dental students and dentists across the globe!

Facebook:
https://www.facebook.com/hackdentistry

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https://www.instagram.com/hack.dentistry/

Twitter:
https://twitter.com/hckdentistry

Muscle Tissues and Sliding Filament Model DrPhil 142 Views • 2 years ago

Join the Amoeba Sisters a they explore different muscle tissues and then focus on the sliding filament theory in skeletal muscle! This video also briefly talks about muscle naming, some vocabulary (such as agonists and antagonists) before focusing on the sliding filament model. Video also mentions general roles of tropomyosin and troponin.
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Table of Contents:
00:00 Intro
0:39 Muscle Tissue Types
1:58 Muscle Characteristics
2:33 Skeletal Muscle Naming and Arrangement
3:26 Actin Myosin and Sarcomere
4:32 Sliding Filament Model
6:55 Tropomyosin an Troponin
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Factual References:

Betts, J. Gordon, et al. “10.3 Muscle Fiber Contraction and Relaxation - Anatomy and Physiology 2e | OpenStax.” Openstax.org, 20 Apr. 2022, openstax.org/books/anatomy-and-physiology-2e/pages/10-3-muscle-fiber-contraction-and-relaxation.

Urry, Lisa A, et al. Campbell Biology. 11th ed., New York, Ny, Pearson Education, Inc, 2017.
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Further Reading Recommendations:

What about I and A bands? What actually initiates the power stroke? How does calcium get released and from where? Remember, there is a lot more detail! We recommend this page from Openstax to learn more:
https://openstax.org/books/bio....logy-2e/pages/38-4-m

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The Amoeba Sisters videos demystify science with humor and relevance. The videos center on Pinky's certification and experience in teaching biology at the high school level. Amoeba Sisters videos only cover concepts that Pinky is certified to teach, and they focus on her specialty: secondary life science. Learn more about our videos here: https://www.amoebasisters.com/our-videos

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TIPS FOR VIEWING EDU YOUTUBE VIDEOS:
Want to learn tips for viewing edu YouTube videos including changing the speed, language, viewing the transcript, etc? https://www.amoebasisters.com/....pinkys-ed-tech-favor

MUSIC:
Our intro music designed and performed by Jeremiah Cheshire.
End music in this video is listed free to use/no attribution required from the YouTube audio library.

COMMUNITY:
We take pride in our AWESOME community, and we welcome feedback and discussion. However, please remember that this is an education channel. See YouTube's community guidelines and how YouTube handles comments that are reported by the community. We also reserve the right to remove comments.

TRANSLATIONS:

Spanish Subtitles Translated by Jeremy García
Hindi Subtitles: Translated by Alisha Aggarwal

We gladly accept subtitle translations from our community. Learn more here: https://www.amoebasisters.com/....pinkys-ed-tech-favor We want to thank our amazing community for the generosity of their time in continuing to create translated subtitles.

We also have videos dubbed in Spanish and Portuguese using an artificial voice via https://aloud.area120.google.com to increase accessibility. See our Amoeba Sisters en Español channel https://www.youtube.com/channe....l/UC1Njo3LBy53cOPngz and Amoeba Sisters em Português https://www.youtube.com/channe....l/UCYTQPX2X_mXe0ZMPi

Respiratory Histology – Histology | Lecturio DrPhil 305 Views • 2 years ago

► Sign up here and try our FREE content: http://lectur.io/freecontentyt
► If you’re an medical educator or faculty member, visit: http://lectur.io/medytb2u

This video “Respiratory Histology” is part of the Lecturio course “Histology” ► WATCH the complete course on http://lectur.io/respiratoryhistology

► LEARN ABOUT:
- The cellular components of epithelium
- Structure and function of the conchae
- The cellular components of olfactory epithelium
- Components of the true vocal cord
- Function of the epiglottis
- The difference between bronchus, bronchiole, respiratory bronchiole
- Alveolar duct and Alveolar sac
- Components that make up the interalveolar septum
- Type I and Type II of alveolar cells, macrophages and endothelium
- Two separate blood supplies to the lungs and their functions
- Summary of the functions and the system of the respiratory system

► THE PROF: Your lecturer is Professor Geoff Meyer. He is currently teaching at the School of Anatomy, Physiology and Human Biology at the University of Western Australia (UWA). As a leading anatomy and histology expert he is also coordinating the Federative International Program for Anatomical Terminologies (FIPAT) of the International Federation of Associations of Anatomists (IFAA). Besides medical research on the ovarian function, steroidogenesis, corpus luteum, angiogenesis, and microcirculation, Geoff Meyer’s research activities also focus on developing innovative, computer-aided learning and teaching tools. For his inventiveness, Geoff Meyer has received a number of awards, including the Australian University Teaching Award.

► LECTURIO is your single-point resource for medical school:
Study for your classes, USMLE Step 1, USMLE Step 2, MCAT or MBBS with video lectures by world-class professors, recall & USMLE-style questions and textbook articles. Create your free account now: http://lectur.io/respiratoryhistology

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Histology of the Airways
http://lectur.io/respiratoryhistoloyarticle

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0:00 Introduction
2:00 Respiratory system Summary of structure and function
3:32 Conducting portion
7:49 The Nasal cavities
11:27 The nasal cavity
13:06 Respiratory mucosa
15:26 Olfactory mucosa
17:13 Olfactory receptor
21:16 The larynx (and the epiglottis)
25:44 The trachea
29:16 The bronchi
31:35 Bronchiole
38:50 Alveolus
40:45 Air blood barrier
43:36 Alveolar macrophages
45:35 Blood supply and lymphatic drainage of the pulmonary lobule

Structure and Types of Cartilage | Hyaline | Elastic | Fibrocartilage | Connective Tissue Histology DrPhil 92 Views • 2 years ago

✨This video is on the structure and functions of the three types of cartilage (Hyaline, Elastic and Fibrocartilage). I hope it helps! ☀️

🌟What's in this video?
0:00 - Intro
0:07 - Connective Tissue Recap
1:16 - Structure of Connective Tissue
1:57 - Structural Components of Cartilage
3:38 - Types of Cartilage
3:49 - Hyaline Cartilage
8:05 - Elastic Cartilage
8:55 - Fibrocartilage

✨ Other videos you may need:
🔅 Connective Tissue : https://youtu.be/xw_ALdt5n-A
🔅 Collagen : https://youtu.be/3e2JYMNS_W4
🔅 Ossification: https://youtu.be/86V9SNWD_No
🔅Histology: https://www.youtube.com/playli....st?list=PL1rG930trF2

💫 For more videos like this, subscribe to my channel!
Byte Size Med: https://youtube.com/channel/UC....ZghvlgylH3r_CWfA18eF

📚Factual References & for Further Reading:
- DiFiore's Atlas of Histology
- Junqueira's Basic Histology
- Gartner's Concise Histology
- Openstax Anatomy and Physiology
https://openstax.org/details/b....ooks/anatomy-and-phy
- Openstax Biology
https://openstax.org/details/books/biology-2e
(The last two are links to open-source references. They are NOT affiliate links)

🌤 Note:
These are just a collection of my notes. So use them the way you would use borrowed notes from a friend. 📝
The images in this video are hand-drawn for illustration and explanation only.✍️ Hence, they may not be anatomically accurate. I am just one person making these videos. If there are any errors, that is unintentional. I try super hard to avoid them. Please let me know if you find any, so it gets clarified for other viewers. Science constantly evolves and changes. New discoveries are made everyday. So some of the information in these videos may become outdated. If you notice that, please let me know so I can update them.

⚡️Disclaimer:
These videos are NOT a substitute for a medical textbook. Textbooks are written by experts (which I do not claim to be), edited, proofread and referenced. Please use them.
The information has been sourced from multiple references as mentioned above. I draw all the pictures myself. But if I have inadvertently infringed on any copyright, that is completely unintentional. I only make these videos to impart education. If I have accidentally violated copyright in any way, do let me know so I can make the necessary changes or give credit to anyone who is owed the same.
These videos are NOT intended for patient education. They are NOT a substitute for diagnosis and treatment by a licensed medical professional. Always seek the advice of a qualified health care provider for any questions you may have regarding any medical condition, so that they can address your individual needs.

🔅They are ONLY meant to help students of medicine and health sciences with studying, and should be used for just that purpose and absolutely nothing else.

Byte Size Med. All Rights Reserved.

Traumatic Brain Injury - Brain Surgery Animation Scott 168 Views • 2 years ago

http://www.highimpact.com - This brain surgery animation was used to demonstrate a young girl's craniotomy, cranioplasty, and reconstructive skull surgery after her vehicle was struck by a tractor-trailer. The procedures included the evacuation of a large epidural hematoma, the draining of the epidural space, and the reassembly of bone fragments to repair the skull.

More Brain Surgery Animations: https://tinyurl.com/y6m4lkdf

WHAT HAPPENED

A teenage girl was riding home with her parents and boyfriend from a Wednesday night church service when a tractor-trailer struck the back driver’s side of their car as they were traveling through an intersection. The impact sent the car spinning into oncoming traffic where it struck another vehicle. When paramedics arrived, the 17-year-old was unresponsive with bleeding from her left ear and a laceration from behind her left ear.

She was rushed to the hospital where she underwent a series of CT scans that showed a severely comminuted open skull fracture with an underlying 1.1 cm subdural hematoma. She was taken to the operating room where an emergency craniotomy was performed to evacuate the hematoma and reassemble the skull fragments. The patient gradually began to wake up and was discharged six days later, after she showed she could maneuver up and down the hallway.

The biggest challenge in a traumatic brain injury case like this - where most of the damages are deeply underlying and undetectable on the surface - is that the only visual evidence is in the form of 2D black-and-white radiographic films. This can look ambiguous to the typical juror because it’s often difficult to discern where these snapshots are located inside the person’s skull. Tony Seaton, Esq., and Robert Bates, Esq., needed to reinforce this 2D radiographic evidence with maximum 3D context.

We equipped them with a custom Diagnostic Slice Chooser: an interactive presentation that presents radiographic slides within a three-dimensional model of the patient’s head. We also designed the model accurately to the patient’s likeness and colorized the films to highlight key areas of damage. The attorneys could show the complete depth and magnitude of his client’s injuries at every level both before and after the surgery. After establishing the full extent of damages, we also created an animation to walk viewers through the surgical experience the patient would undergo as a result of her injuries.

The visual presentation helped jurors understand the destructive impact this collision had on this young teenager’s life, and Mr. Seaton and Mr. Bates, Esq., were able to acquire a $4.5M settlement for his client.

Read the Full Case Study: https://tinyurl.com/yy4v2dyh

Ventriculostomy Brain Surgery - 3d animation Scott 800 Views • 2 years ago

This 3D animation of brain surgery, shows how a ventriculostomy is performed, which is a neurosurgical procedure of creating a hole within a cerebral ventricle for drainage. It is most commonly performed on those with hydrocephalus, an abnormal buildup of fluid in the ventricles (cavities) deep within the brain. It's done by surgically penetrating the skull, dura mater, and brain such that the ventricular system ventricle of the brain is accessed.

When catheter drainage is temporary, it is commonly referred to as an external ventricular drain (EVD). When catheter drainage is permanent, it is usually referred to as a shunt.

There are many catheter-based ventricular shunts that are named for where they terminate, for example, a ventriculi-peritoneal shunt terminates in the peritoneal cavity, a ventriculoarterial shunt terminates within the atrium of the heart, etc. The most common entry point on the skull is called Kocher's point. An EVD ventriculostomy is done primarily to monitor the intracranial pressure as well as to drain cerebrospinal fluid (CSF), primarily, or blood to relieve pressure from the central nervous system (CNS).

For more information about custom medical animation, please visit https://www.amerra.com/.

Watch additional medical animations:

Craniectomy brain surgery - 3D animation: https://youtu.be/1RkseDeYS9g

Accessing an implantable port training - 3D animation: https://youtu.be/xSTpxjyv4O4

Open Suctioning with a Tracheostomy Tube - 3D animation: https://youtu.be/wamB7jpWCiQ

Suctioning the endotracheal tube - medical animation: https://youtu.be/pN6-EYoeh3g

Functional endoscopic sinus surgery (FESS) - 3D animation: https://youtu.be/qKTRyowwaLA

How to insert a nasogastric tube for NG intubation - 3d animation: https://youtu.be/Abf3Gd6AaZQ

Oral airway insertion - oropharyngeal airway technique - 3D animation: https://youtu.be/caxUdNwjt34

Nasotracheal suctioning (NTS) - 3D animation: https://youtu.be/979jWMsF62c

Learn about hemorrhoids with #3d #animation: https://youtu.be/R6NqlMpsiiY

LASIK eye surgery - 3D animation: https://youtu.be/Bb8bnjnEM00

CPR cardiopulmonary resuscitation - 3D animation: https://youtu.be/G87knTZnhks

What are warts (HPV)? - 3D animation: https://youtu.be/guJ1J7rRs1w

How Macular Degeneration Affects Your Vision - 3D animation: https://youtu.be/ozZQIZ_52YY

NeoGraft hair transplant procedure – animation: https://youtu.be/C-eTdH2UPXI

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