The most popular and one of the principal stains in histology is hematoxylin and eosin stain. It gives us an overview of the tissue and its structure. Hematoxylin binds with basophilic structures – for example DNA and RNA. So we can observe nuclei stained in blue or purple color. Eosin binds to acidophilic substances such as positively charged amino acid side chains. So as the result cytoplasm is pink or orange. All samples in laboratory are stained with H&E. There are several different types of hematoxylins and eosins used in histology which will give us different results.

In this video you will see, how we stain slides with different types of hematoxylins and eosins. Finally, we will compare the results.

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Tibial Bone Transport Over an Intramedullary Nail Using Cable and Pulleys

The spleen is one of the most frequently injured intraperitoneal organs, and management of splenic injuries may require splenectomy .. The spleen is an wedge-shaped organ that lies in relation to the ninth and 11th ribs, located in the left hypochondrium and partly in the epigastrium; thus, it is situated between the fundus of the stomach and the diaphragm. The spleen is highly vascular and reddish purple; its size and weight are variable. A normal spleen is not palpable. The spleen's key function is the removal of old red blood cells "RBCs", defective circulating cells, and circulating bacteria. In addition, the spleen helps maintain normal erythrocyte morphology by processing immature erythrocytes, removing their nuclei, and changing the shape of the cellular membrane. Other functions of the spleen include the removal of nuclear remnants of RBCs, denatured hemoglobin, and iron granules ..

High volume sinus irrigation!

Epilepsy surgery is reserved for people whose seizures are not well controlled by seizure medicines. This situation is sometimes called being "medically refractory" or "drug resistant." In children, the definition of medically refractory is even more individualized to the specific child's situation. Surgery may be considered for some children after weeks to months of treatment with seizure medicines.

Open Appendectomy Surgery Video

Breast Cancer spreads by 3 mechanisms- local spread, by lymph nodes, or through the blood. Dr. Lorraine Champion, and Dr. Lisa Bailey discuss how breast cancer spreads. They discuss the different methods of spread and how this will affect the treatment of breast cancer.

Tension pneumothorax describes the progressive accumulation of air in the pleural cavity (normally a potential space) through a defect in the visceral pleura. This leads to positive pressure being maintained and increasing throughout the respiratory cycle causing vessels within the mediastinum to be compressed with catastrophic consequences if left untreated. Clinical signs include hypoxia, hypotension, tachycardia, reduced breath sounds and hyper resonance ipsilaterally, with tracheal deviation (away from the affected side) and distended neck veins being late clinical signs.

The ureter can become obstructed due to conditions such as kidney stones, tumors, infection, or blood clots. When this happens, physicians can use image guidance to place stents or tubes in the ureter to restore the flow of urine to the bladder. A ureteral stent is a thin, flexible tube threaded into the ureter.

Treatment may not be needed for an eschar if it is part of the natural healing process. However, if an eschar looks like it may have a wound infection – symptoms can include oozing fluid such as pus or blood, your clinician will likely recommend topical treatment or debridement to help control and remove the infection.

Healthcare providers are in the best position to assess for domestic violence, yet have obstacles to doing so. See the benefits to moving beyond these obstacles for those you serve. And discover an accurate, convenient and confidential way to assess for domestic abuse.

Watch that video of Removing Gauze From a Spider's Bite

CRT is a clinically proven treatment option for some individuals with heart failure. A CRT device sends small electrical impulses to both lower chambers of the heart to help them beat together in a more synchronized pattern. This may improve the heart’s ability to pump blood and oxygen to your body. A CRT system is made up of two parts. The heart device, which is actually a tiny computer, plus a battery, contained in a small titanium metal case that is about the size of a pocket watch. Insulated wires, called leads, that are implanted to carry information signals from your heart to the heart device and to carry electrical impulses to your heart After the device system is implanted, an external computer, called a programmer, located at your doctor's office or clinic can be used to program the heart device and retrieve information from your heart device that will assist your doctor in your heart failure treatment. Your doctor will schedule periodic monitoring which may be done remotely if physician deems appropriate

This anatomical implant was originally placed in 1997. Due to the dark yellow color inside the implant it is clear the implant has been ruptured for quite some time. When implants rupture, it is important to have them replaced as soon as possible to avoid excessive scarring in the breasts. If too much scar tissue has accumulated around the deflated implant, it becomes difficult to create a normal breast shape in the future. Therefor its important to know the signs of a ruptured implant such as, painful to touch, visible asymmetry or loss of integrity to the bag. Dr. Stuart Linder 9675 Brighton Way Suite 420 Beverly Hills, CA 90210

How to Remove Blackhead from the Face

watch that video of Navel stone removal from a dirty bellybutton

Intramedullary nailing of the tibia with suprapatellar entry and semi-extended positioning makes it technically easier to nail the proximal and distal fractures. The purpose of this article was to describe a simple method for suprapatellar nailing (SPN). A step-by-step run through of the surgical technique is described, including positioning of the patient. There are as yet only a few clinical studies that illustrate the complications with this method, and there has been no increased frequency of intraarticular damage. Within the body of the manuscript, information is included about intraarticular damage and comments with references about anterior knee pain.

Retinitis pigmentosa is a rare, inherited degenerative eye disease that causes severe vision impairment. Symptoms often begin in childhood. They include decreased vision at night or in low light and loss of side vision (tunnel vision). There's no effective treatment for this condition. Wearing sunglasses may help protect remaining vision.

Fibroadenomas (fy-broe-ad-uh-NO-muhz) are solid, noncancerous breast tumors that occur most often in adolescent girls and women under the age of 30. You might describe a fibroadenoma as firm, smooth, rubbery or hard with a well-defined shape. Usually painless, a fibroadenoma might feel like a marble in your breast, moving easily under your skin when touched. Fibroadenomas vary in size, and they can get bigger or even shrink on their own. Fibroadenomas are among the most common breast lumps in young women. Treatment may include monitoring to detect changes in the size or feel of the fibroadenoma, a biopsy to evaluate the lump, or surgery to remove it.